Figure 4.

TGFBR2/Smad3 is the direct target of miR-145. (A) TGFBR2 and Smad3 was identified as downstream targets of miR-145. (B) T24 cells were treated with celecoxib (60 or 80 µM) or transfected with miR-145 mimic (20 or 40 nM) for 24 h, and the expression levels of TGFBR2 and Smad3 were examined using a reverse transcription-quantitative polymerase chain reaction. GAPDH was used as an internal control. (C) Protein expression levels of TGFBR2 and Smad3 in T24 cells were detected using western blot analysis. GAPDH was used as an internal control. ^*P<0.05 vs. the DMSO or miR-NC mimic group. ^**P<0.05 vs. DMSO and celecoxib (60 µM) or miR-NC mimic and miR-145 mimic (20 nM) group. Smad3, SMAD family member 3; miR, microRNA; TGFBR2, transforming growth factor β receptor 2; DMSO, dimethyl sulfoxide; NC, negative control.