Table I.
Oligonucleotide sequences for reverse transcription and polymerase chain reaction amplification.
| Gene | Sequence (5′-3′) | |
|---|---|---|
| Vimentin | F: ACCGCACACAGCAAGGCGAT | R: CGATTGAGGGCTCCTAGCGGTT |
| E-cadherin | F: AGCTGCCCAGAAAATGAAAAAGG | R: GTGTATGTGGCAATGCGTTCTC |
| Transforming growth factor β receptor 2 | F: CTAACCTGCTGCCTGTGTGA | R: TCTGGAGCCATGTATCTTGC |
| SMAD family member 3 | F: CCATCTCCTACTACGAGCTGAA | R: CACTGCTGCATTCCTGTTGAC |
| GAPDH | F: CATGAGAAGTATGACAACAGCCT | R: AGTCCTTCCACGATACCAAAGT |
| microRNA-145 | F: GTCCAGTTTTCCCAGG | R: GAGCAGGCTGGAGAA |
| RT primer: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAGAACAGT | ||
| U6 | F: CGCAAGGATGACACG | R: GAGCAGGCTGGAGAA |
| RT primer: CGCTTCACGAATTTGCGTGTCAT |
F, forward; R, reverse; RT, reverse transcription.