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. 2019 May 30;216(7):1582–1598. doi: 10.1084/jem.20181895

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© 2019 Hisano et al.

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Figure 3. — LPAR1-mediated S1PR1/β-arrestin coupling in G protein–deficient cells. LPAR1 or empty vector was transfected with S1PR1-SmBiT and LgBiT-β-arrestin1 into HEK293 fullΔGα cells lacking all Gαs. (A and B) Luminescence was measured 15–20 min after S1P (A) or LPA (B) stimulation. Experiments were repeated (i.e., n = 3 independent experiments), and results are expressed as mean ± SD. βARR, β-arrestin; RLU, relative light units.