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. 2019 Jun 28;11:5745–5756. doi: 10.2147/CMAR.S197455

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© 2019 Xiong et al.

This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).

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Deep sequencing performance. 107 DNA samples derived from 33 HCC tissue specimens, 37 peripheral blood samples, and matched 37 swab samples were subject to deep sequencing, and the performance was evaluated. (A) The distribution of the sequenced fragments of the plasma cfDNA. Three main peaks with lengths of 160 bp, 320 bp, and 480 bp were identified. (B) The distribution of the sequenced fragments of the DNA samples from the HCC tissue specimens. The average read length was approximately 300 bp. The sequencing depths for (C) the plasma cfDNA and (D) the HCC tissue DNA. (E) The background rates. SNVs and germline SNPs were excluded, and the mean and median background rates were calculated.

Abbreviation: HCC, hepatocellular carcinoma.