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. 2019 Jun 11;218(7):2169–2184. doi: 10.1083/jcb.201902143

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© 2019 Matson et al.

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Figure 5. — Cells re-entering the first G1 after G0 lack a licensing checkpoint-induced G1 delay. (A) Immunoblot of total protein lysates of cells released from G0 into the first cell cycle with siControl or siCdt1 for 24 h and of proliferating cells treated with siControl or siCdt1 for 72 h. (B) Mean cell cycle phase distribution of cells treated as A defined by flow cytometry (DAPI and EdU). n = 3, mean with SD. (C) Immunoblot of total protein lysate of RPE1 WT and p53 KO synchronized in G0 or released into the first cell cycle with siControl or siCdt1 for 24 h as indicated. (D) Cell cycle phase distribution of cells treated as in C defined by flow cytometry. n = 3, mean with SD.