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Effects of co-culture of A549 cells and macrophages on A549 cells.
(a, b) CD163 and CD68 proteins and mRNAs were tested by Western blot and qRT-PCR. (c) CCK-8 assay was used to tested the cell viability. (d-f) KLF6-SV1, TWIST1 and CCL2 proteins and mRNAs were tested by using Western blot and qRT-PCR. (g, h) ELISA was applied to detect the M-CSF and GM-CSF expressions. *P < 0.05,**P < 0.01, versus control group.
