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. 2019 Mar 27;20(6):729–739. doi: 10.1080/15384047.2018.1529121

Figure 4.

Figure 4.

MSC-AS1 serves as a ceRNA for miR-29b-3p to counteract miR-29b-mediated repression of CDK14 (a) miR-29b-3p expression was achieved by transfection of miR-29b-3p mimics or miR-29b-3p inhibitor into Panc-1 and BxPC-3 cells, as confirmed using real-time PCR. (b) Expression of MSC-AS1 in response to miR-29b-3p overexpression or miR-29b-3p inhibition was examined in Panc-1 and BxPC-3 cells. (c) Protein levels of CDK14 in response to miR-29b-3p overexpression or miR-29b-3p inhibition was examined in Panc-1 and BxPC-3 cells. (d) The interaction between miR-29b-3p and CDK14, between miR-29b-3p and MSC-AS1 was validated by luciferase reporter gene assays. (e) Association of miR-29b-3p and MSC-AS1 with AGO2 in Panc-1 and BxPC-3 cells. Detection of AGO2 using Immunoblotting assays. (f) RIP assay in Panc-1 and BxPC-3 cells transfected with vector-NC (negative control) or MSC-AS1 overexpressing vector followed by real-time PCR to detect MSC-AS1 and CDK14 associated with AGO2. The data are presented as mean ±SD of three independent experiments. *P < 0.05, **P < 0.01.