. 2019 Feb 20;20(6):866–876. doi: 10.1080/15384047.2019.1579957

Table 1.

PCR primers for truncated fragments of cagA, vacA and babA genes of H. pylori.

Gene fragment	Primer pair	Sequence	Gene loci (nt)	Target length
c-1	c1-f	5ʹ-GGTACCATGACTAACGAAGCCAT-3’	1–17	1367 bp
c-1	c1-r	5ʹ-CCTAGGGAAACAAAAGCAATGTGAT-3’	1337–1355	1367 bp
c-2	c2-f	5ʹ-GGTACCATGATCACATTGCTTTTGTTTC-3’	1337–1355	1253 bp
c-2	c2-r	5ʹ-CCTAGGAGATAACCCATTACCGACTA-3’	2555–2574	1253 bp
c-3	c3-f	5ʹ-GGTACCATGGTTATCTAAAGCAGAAGCC-3’	2568–2586	950 bp
c-3	c3-r	5ʹ-CCTAGGAAGATTTTTGGAAACCACCTTT-3’	3481–3502	950 bp
v-1	v1-f	5ʹ-CCTAGGATGGCGGTGTCAATCTGTCCAA −3’	1274–1291	1283bp
v-1	v1-r	5ʹ-CTCGAGATTAGTGGTGTTTGTGGGTAAGT-3’	2519–2541	1283bp
v-2	v2-f	5ʹ-CCTAGGATGACCAACTTACCCACAAACAC-3’	2515–2534	1331bp
v-2	v2-r	5ʹ-CTCGAGTGGCTTGCGTTGGAAATCAA-3’	3811–3830	1331bp
b-1	b1-f	5ʹ-GGTACCATGAAAAAACACATCCTT- 3’	1–18	1325bp
b-1	b1-r	5ʹ-CCTAGGGTAACTGTGCCTGGAGC- 3’	1297–1313	1325bp
b-2	b2-f	5ʹ-GGTACCATGGCTCCAGGCACAGTTAC-3’	1297–1313	953bp
b-2	b2-r	5ʹ- CCTAGGTAATAAGCGAACACGTA- 3’	2218–2234	953bp

Note: The underlined oligo nucleotides are Kpn I (GGTACC), BamH I (CCTAGG), Xho I (CTCGAG) enzyme sites, respectively. Abbreviations: f, forward primer; r, reverse primer; nt, nucleotide; bp, base pair.