Figure 6.

Lamc1 suppresses the cell proliferation and Warburg effect via regulating PKM2 (a,b). The efficiency of oePKM2 regulating PKM2 expression in HepG2 cells was determined by RT-PCR (a) and western blot (b). After co-infection of lentiviruses siLamc1 and oePKM2 in HCC cells, (c) the cell proliferation were assessed by CCK8 assays at 0, 24, 48 and 72 h. (d) The cell death rates in HepG2 were evaluated by Trypan blue staining. (e,f) The glucose consumption (e) and lactate production (f) of HCC cells were detected by biochemical detections. (g) The protein levels of PKM2, GLUT1 and LDHA were detected by western blot. With at least three independent experiments, the data were expressed as mean ± SD, **P < 0.01 and ***P < 0.001 compared with Vector, ^##P < 0.01 and ^###P < 0.001 compared with siLamc1.