Figure 2.

Silencing of LINC00994 inhibits the growth of pancreatic cancer cells.

(a) The basal levels of LINC00994 in sw1990, AsPC-1 and Panc-1 were determined with real-time qPCR. LINC00994 exclusive shRNA was used to stably knock down the endogenous LINC00994 in Panc-1 (b) and AsPC-1 cells (c). (d-e) Cell growth was evaluated by staining the proliferative cancer cells with CCK-8 reagent (N = 5). (f-g) Cell cycle process was determined by staining cancer cells with PI, and analyzed via flow cytometry (N = 3). ^{#, ##, ###} denoted P < 0.05, <0.01, and <0.001, respectively. nc, negative control