Figure 5.

LINC00994 expression is negatively correlated with miR-765-3p in pancreatic cancers.

MiRNA expression profiles were obtained from pancreatic tumor and non-tumor tissues derived from three patients via microarray. All analyzed miRNAs were plotted in part (a). Nine downregulated and three upregulated miRNAs (fold change ≥ 2 and P value < 0.05) were further analyzed with unsupervised hierarchical clustering, and presented in a heat map (b). (c) Real-time qPCR was performed to analyze the levels of LINC00994 and miR-765-3p in 10 paired pancreatic samples. (d) The expression correlation of LINC00994 and miR-765-3p was analyzed with GraphPad prism V7. (e) The protein expression levels of RUNX2, a target of miR-765-3p, were determined with western blotting. ^{#, ##, ###} denoted P < 0.05, <0.01, and <0.001, respectively. nc, negative control