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. 2019 Mar 5;20(6):922–930. doi: 10.1080/15384047.2019.1579962

Figure 2.

Figure 2.

Transcriptional regulation by CerS6. HEK293 cells were co-transfected with 100ng of each plasmid and luciferase reporter activity quantified 24 hours post-transfection. (a) control and CerS6 shRNA co-transfected with SNAI1-luc reporter, (b) control and CerS6 shRNA co-transfected with SNAI2-luc reporter, (c) empty and SNAI2 expressing vector co-transfected with CerS6-luc reporter. Data are from 3 independent experiments each performed in triplicate. (d) Western blot of HEK293 cells transfected with SNAI2 showing lack of impact on CerS6 expression. Actin serves as loading control.