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Sphingolipid profiles of SW480 cells following treatment with C6-ceramide nanoliposomes. SW480 cells expressing CerS6 (a) or with CerS6 knockdown (b) were incubated with 50μM ghost nanoliposomes or 25μM and 50μM C6-ceramide nanoliposomes. At 36 hours cells were collected for LC/MS analysis. Data shown are the average and standard deviation from two independent experiments each performed in triplicate.
