Table 1.
Sphingolipid composition in SW480 with and without CerS6 expression. SW480 cells were analyzed for sphingolipids by LC/MS. Data is the average and standard deviation from 2 independent experiments each performed in triplicate expressed as pmol sphingolipid per nmol total phosphate (pmol SL/nmol Pi). Other ceramides includes C14, C18, C18:1, C20, C22:1 and C26:1, which were each 1% of total ceramide or less.
| CerS6 expressed |
CerS6 knockdown |
|||
|---|---|---|---|---|
| Sphingolipid | pmol SL/ nmol Pi | % of total ceramide | pmol SL/ nmol Pi | % of total ceramide |
| C16-ceramide | 2.4078 ± 0.2954 | 28 ± 4 | 0.9959 ± 0.0146*** | 19 ± 5*** |
| C22-ceramide | 0.5706 ± 0.0362 | 7 ± 2 | 0.378.6 ± 0.0558*** | 7 ± 0 |
| C24-ceramide | 3.3829 ± 0.4618 | 39 ± 4 | 2.3480 ± 0.4853** | 43 ± 1 |
| C24:1 ceramide | 2.0166 ± 1.1361 | 22 ± 8 | 1.4731 ± 0.4265 | 27 ± 3 |
| C26-ceramide | 0.1093 ± 0.0127 | 1 ± 0 | 0.0451 ± 0.0152*** | 1 ± 0 |
| other ceramides | 0.0268 ± 0.0113 | 3 ± 1 | 0.0214 ± 0.0091 | 4 ± 1 |
| dhC16-ceramide | 0.3093 ± 0.0239 | 0.0804 ± 0.0158*** | ||
| sphingosine | 0.0794 ± 0.0099 | 0.0389 ± 0.0035*** | ||
| S1P | 0.0044 ± 0.0014 | 0.0031 ± 0.0008 | ||
| Total ceramide | 8.7549 ± 1.9267 | 5.4554 ± 0.9953** | ||
p < 0.01,* p < 0.001, ***p < 0.0001.