Skip to main content
. 2019 Mar 31;20(7):1007–1016. doi: 10.1080/15384047.2019.1595276

Figure 2.

Figure 2.

Knockdown of FOXD2-AS1 inhibited HCC cell proliferation and induced HCC cell apoptosis. A. The expression level of FOXD2-AS1 in one normal cell line and four HCC cell lines was tested. B. FOXD2-AS1 was silenced in HepG2 and LM3 cells by specific shRNA (termed sh-FOXD2-AS1). The transfection efficiency was examined with the qRT-PCR examination. C-D. MTT and trypan blue exclusion were carried out to determine the cell viability in HCC cells transfected with sh-FOXD2-AS1 or sh-NC. E. Colony formation assay was performed to test the proliferative ability of HepG2 and LM3 cells transfected with sh-FOXD2-AS1 or sh-NC. F. Flow cytometry analysis was then utilized to assess cell apoptosis. *P < 0.05, **P < 0.01 vs control group.