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. 2019 Mar 31;20(7):1007–1016. doi: 10.1080/15384047.2019.1595276

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Figure 5. — FOXD2-AS1 enhanced the activity of Wnt/β-catenin signaling pathway. A. The protein levels of the core factors of PI3K/AKT signaling pathway and Wnt/β-catenin signaling pathway were examined in HepG2 and LM3 cells transfected with sh-FOXD2-AS1. B. The viability of HepG2 cell treated with a different dose of LiCl was evaluated with MTT assay. C. The proliferative ability of cell treated with sh-FOXD2-AS1 and LiCl was examined with colony formation assay. D. The cell apoptosis increased by FOXD2-AS1 knockdown was partly decreased by LiCl. E. The migratory ability of cell treated with sh-FOXD2-AS1 and LiCl was detected with transwell migration assay. F. The reversal effect of silenced FOXD2-AS1 on the EMT progress was attenuated by LiCl. *P < 0.05, **P < 0.01 vs control group.