Figure 3.

ZCL367 inhibits Cdc42 activation downstream of GM130. Swiss 3T3 cells were starved ON then preincubated with ZCL367 (50 μM) for 2 h. Bradykinin (100 ng/mL) was used to activate Cdc42/Rac1 for 20 m. Cells were fixed and stained for GM130 (red), nucleus (blue), and phalloidin (green). GM130 organization was conserved in ZCL367 treated cells. Activation with bradykinin resulted in altered distribution of GM130. Treatment with ZCL367 inhibited filopodia formation but did not affect GM130 distribution. Cells that showed strong Rac1 activation in the presence of ZCL367 showed a GM130 staining similar to control.