Figure 4.

β₂m knockdown by siRNA reduces the APLP2 expression level in S2-013 and PANC-1 pancreatic cancer cells, elevates APLP2 expression in MIA PaCa-2 pancreatic cancer cells, and does not significantly change the level of APLP2 in non-transformed hTERT-HPNE pancreatic cells. The indicated cell lines were cultured with β₂m siRNA (or scrambled sequence control siRNA) for 72 h and cell lysates were collected and tested by Simple Western for β₂m (to verify β₂m knockdown by siRNA) and immunoblotted with an antibody for APLP2 or (as the control) Hsc70. The APLP2-specific antibody recognizes both APLP2 and the high molecular weight GAG-modified form of APLP2. The results shown are representative of 5 independent experiments for S2-013, PANC-1, and MIA PaCa-2, and of 2 independent experiments for hTERT-HPNE.