Figure 5.

β₂m knockdown by siRNA causes a deficiency in HLA class I heavy chain expression in all three pancreatic cancer cell lines tested. S2-013, PANC-1, and MIA PaCa-2 cells were cultured with β₂m siRNA (or scrambled sequence control siRNA) for 72 h and cell lysates were collected. The lysates were tested for β₂m by Simple Western (to verify β₂m knockdown by siRNA) and immunoblotted for the HLA class I heavy chain (with antibody HC10) or for a control protein (actin for S2-013 and Hsc70 for PANC-1 and MIA PaCa-2). The results shown are representative of 8 independent experiments for S2-013 and 3 separate experiments for PANC-1 and MIA PaCa-2.