Key resources table.
| Reagent type (species)or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strainbackground (M. musculus) |
C.B-17 SCID beige mice | Charles River Labs | CB17.Cg-PrkdcscidLystbg-J/Crl | |
| Strain, strainbackground (M. musculus) |
Athymic nude mice | Harlan Sprague Dawley (Livermore facility) | Hsd:Athymic Nude-Foxn1nu | |
| Genetic reagent (M. musculus) |
Lgr5CreERT2+; tdTomato; KrasG12Dwt/ki; PTENloxP/loxP |
Lgr5CreERT2+; pubmed id:21927002
tdTomato; pubmed id: PMC2840225 KrasG12Dwt/ki; pubmed id: 11751630 PTENloxP/loxP; pubmed id: 11691952 |
LAR, Genentech | |
| Antibody | Anti-Ragweed | Genentech | 9652 | In vivo: 20 mg/kg, i.p.,qwk In vitro: 20 ug/ml |
| Antibody | Anti-NRG1 | Genentech | YW538.24.71 | In vivo: 20 mg/kg, i.p.,qwk In vitro: 20 ug/ml |
| Antibody | deltaNTP63 (Rabbit Polyclonal IgG) | Biolegend | RRID:AB_2256361
Cat. #: 619001 |
WB (1:1000) |
| Antibody | Actin (Mouse IgG) | BD Bioscience | RRID:AB_2289199
Cat. #: 612656 |
WB (1:5000) |
| Antibody | p-ERBB3 (Rabbit mAb) | Cell Signaling Technologies | RRID:AB_2099709
Cat. #: 4791 |
WB (1:500) |
| Antibody | ERBB3 (Rabbit mAb) | Cell Signaling Technologies | RRID:AB_2721919
Cat. #: 12708 |
WB (1:500) |
| Antibody | Rabbit PARP (Rabbit polyclonal) | Cell Signaling Technologies | RRID:AB_2160739
Cat. #: 9542 |
WB (1:1000) |
| Antibody | Cleaved caspase-3 (Rabbit mAb) | Cell Signaling Technologies | Cat. #: 9664 | WB (1:1000) |
| Antibody | TP63 alpha (Rabbit mAb) | Cell Signaling Technologies | RRID:AB_2637091
Cat. #: 13109 |
CHIP (1:100) WB (1:1000) |
| Antibody | KRT10 (Rabbit polyclonal) | Covance Biologicals | RRID:AB_291580
Cat. #: PRB-159P |
IHC (1:1000) |
| Commercial assay or kit | TP63-all | Life Technologies | Hs00978340_m1 | qPCR |
| Commercial assay or kit | TA-TP63-TA | Life Technologies | Hs00186613_m1 | qPCR |
| Commercial assay or kit | deltaN-TP63 | Life Technologies | Hs00978339_m1 | qPCR |
| Commercial assay or kit |
NRG1a | Life Technologies | Hs01103794_m1 | qPCR |
| Commercial assay or kit | NRG1b | Life Technologies | Hs00247624_m1 | qPCR |
| Commercial assay or kit | KRTDAP | Life Technologies | Hs00415563_m1 | qPCR |
| Commercial assay or kit | KRT1 | Life Technologies | Hs00196158_m1 | qPCR |
| Commercial assay or kit | KRT10 | Life Technologies | Hs00166289_m1 | qPCR |
| Commercial assay or kit | IVL | Life Technologies | Hs00846307_s1 | qPCR |
| Other | TA-TP63 | Dharmacon | #13 = J-003330–13 | siRNA |
| Other | all isoform-TP63 | Dharmacon | #14 = J-003330–14 | siRNA |
| Other | NTC#3 | Dharmacon | D-001810–03 | siRNA |
| Other | NTC#4 | Dharmacon | D-001810–04 | siRNA |
| Software,algorithm | GraphPad Prism | graphpad.com | RRID:SCR_002798 |