Table 1. LC-MS quantification of cohesin subunit copy number.

Absolute quantification of cohesin subunits in chromatin or soluble extracts isolated from G1, G2 or prometaphase synchronised HeLa cells, adjusted for cell number. Data are tabulated as [mean - s.d., mean + s.d.] of two biological replicates and two technical replicates (s.d. = standard deviation). Values in bold or italics derive from quantification of one peptide or two peptides, respectively. Underlined values derive from quantification of a single biological replicate. For individual peptide counts, see Figure 1—figure supplement 2.

	G1		G2		Prometaphase
Protein	Chromatin-bound	Soluble	Chromatin-bound	Soluble	Chromatin-bound	Soluble
SMC1	61125 [47609, 74640]	-	142290 [120274, 164306]	-	12461 [11497, 13425]	-
SMC3	66430 [53421, 79440]	350228 [312360, 388095]	158478 [129846, 187111]	622117 [557712, 686522]	14165 [12173, 16158]	899510 [816643, 982376]
SCC1	69275 [54577, 83972]	281140 [199837, 362444]	149500 [127373, 171627]	356850 [270483, 443217]	13973 [10478, 17468]	494155 [314240, 674070]
STAG1	14718 [11295, 18140]	114355 [104557, 124152]	23017 [17947, 28087]	120960 [119860, 122061]	2797 [1842, 3752]	152033 [147488, 156577]
STAG2	44812 [37745, 51879]	144160 [131233, 157086]	106106 [93591, 118621]	235980 [213879, 258081]	8112 [6863, 9361]	336062 [305203, 366922]