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. 2019 Feb 22;20(6):886–896. doi: 10.1080/15384047.2019.1579959

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Figure 5. — KCNQ1OT1 positively modulated the expression of ZEB1 through sponging miR-217.

(a) Nuclear separation experiment was used to determine the location of KCNQ1OT1 in cytoplasm and nucleus. (b) Seven miRNAs bound with ZEB1 were found through searching from bioinformatics websites (PITASites and miRandaSites). (c,d) MS2-RIP assay was performed to validate the interaction between these seven miRNAs and KCNQ1OT1. (e) Ago2-RIP assay further validated the interaction between KCNQ1OT1 and miR-217. (f) Pull down assay was further applied to demonstrate the interaction between KCNQ1OT1 and miR-217. (g) The binding sites harboring the miR-217 for KCNQ1OT1 were obtained from online bioinformatics analysis. (h) Luciferase activity reporter assays were applied to determine the interaction between KCNQ1OT1 and miR-217. N.S: no significance, **P < 0.01 vs. control group.