Figure 6.

ZEB1 is a target of miR-217 in CRC cells.

(a) Pull-down assay was used to validate whether ZEB1 can be efficiently pulled down by biotin-labeled miR-217. (b) The binding sites between miR-217 and ZEB1 were predicted and obtained using bioinformatics analysis. (c) Dual luciferase reporter assay was applied to demonstrate the binding of KCNQ1OT1 to miR-217. (d) ZEB1-WT vector and empty vector were inserted into HEK-293T cell by co-transfecting with miR-NC, miR-217 mimics or miR-217 mimics + pcDNA-KCNQ1OT1. (e,f) qRT-PCR and western blot assays were performed to test the levels of EMT markers in DLD1 and SW480 cells which were transfected with miR-217 mimics or co-transfected with miR-217 mimics and pcDNA-KCNQ1OT1. N.S: no significance, *P < 0.05, **P < 0.01 vs. control group.