Figure 7.

ZEB1 transcriptionally activated KCNQ1OT1 in CRC cells.

(a) The binding sequences of ZEB1 to the promoter region of KCNQ1OT1 were predicted from JASPAR. (b) Luciferase reporter vectors containing serial truncations of KCNQ1OT1 promoter were constructed and transfected into HEK-293T cells. The luciferase activity of these plasmids was measured. (c) ChIP assay was performed to demonstrate the direct affinity of ZEB1 to the promoter region of KCNQ1OT1 in two CRC cells. (d) ZEB1 was efficiently overexpressed or silenced in DLD1 and SW480 cells by transfecting with sh-ZEB1 and ZEB1 expression vector. (e,f) The expression level of KCNQ1OT1 in DLD1 and SW480 cells was examined in response to the knockdown or overexpression of ZEB1. *P < 0.05, **P < 0.01 vs. control group.