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. 2019 Feb 22;20(6):886–896. doi: 10.1080/15384047.2019.1579959

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Figure 8. — KCNQ1OT1-miR-217-ZEB1 feedback loop regulated the proliferation, migration and EMT formation of CRC cells.

(a,b) MTT assay and colony formation assay were carried out to detect the proliferation ability of DLD1/sh-KCNQ1OT1 cells after transfection with miR-217 inhibitors or co-transfection with miR-217 inhibitors + sh-ZEB1. (c) Transwell assay revealed the metastatic ability of DLD1/sh-KCNQ1OT1 cells after transfection with miR-217 inhibitors or co-transfection with miR-217 inhibitors + sh-ZEB1. (d) The levels of EMT markers were examined in indicated DLD1 cells after transfections. **P < 0.01 vs. control group.