Figure 1.

Acquisition of porcine iNKT cells and conventional αβ T cells (Tconv) for TCR sequencing. Peripheral blood mononuclear cells (PBMCs) were labeled with PBS57-loaded or unloaded CD1d tetramer and anti-CD3 antibody seven days after culture with vehicle (DMSO) or α-GalCer. PBMCs cultured with α-GalCer were FACS sorted into iNKT cell (CD3ε⁺CD1d tetramer⁺) and Tconv (CD3ε⁺CD1d tetramer⁻) populations to a purity of >90% according to the post-sort analysis.