| Component | Amount |
| Milli-Q water | To final volume of 50 μL |
| Sheared genomic DNA of interest (from Subheading 3.4) | 300 ng |
| Sheared CpG methylated lambda DNA (from Subheading 3.3.1) | 2.5 ng |
| Tet oxidation reagent 2 | 15 μL |
| Tet oxidation reagent 1 | 3.5 μL |
| mTet1 protein (5 mg/mL) | 4 μL |
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