| Initial denaturation | 95 °C | 2 min |
| Denaturation | 95 °C | 30 s |
| Annealing | 51 °C | 30 s |
| Extension | 72 °C | 1 min |
| 40 cycles | ||
| Final extension | 72 °C | 7 min |
| Hold | 4 °C |
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