Tet-Assisted Bisulfite Sequencing (TAB-seq)

. Author manuscript; available in PMC: 2019 Jul 2.

Published in final edited form as: Methods Mol Biol. 2018;1708:645–663. doi: 10.1007/978-1-4939-7481-8_33

Component	Amount
Treated genomic DNA (300–600 ng)	Variable depending on its concentration
dNTP mix (10 mM each dNTP)	5 μL
10× End-Repair Buffer	5 μL
ATP (10 mM)	5 μL
End-Repair Enzyme Mix	1 μL
Milli-Q water	To a final volume of 50 μL