| Component | Amount |
| Bisulfite-converted DNA (from step 12) | 7 μL |
| Library primer-F (10 μM) | 3 μL |
| Library primer-R (10 μM) | 3 μL |
| 2× KAPA HiFi HotStart ReadyMix | 25 μL |
| Milli-Q water | To a final volume of 50 μL |
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