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. Author manuscript; available in PMC: 2019 Jul 2.
Published in final edited form as: Methods Mol Biol. 2018;1708:645–663. doi: 10.1007/978-1-4939-7481-8_33
Component Amount
10× PfuTurbo Cx reaction buffer 5 μL
dNTP Mix (10 mM each dNTP) 1 μL
5mC test primer-F (10 μM) 1 μL
5mC test primer-R (10 μM) 1 μL
Bisulfite-treated DNA (from step 5) 2–4 μL
PfuTurbo Cx Hotstart DNA polymerase (2.5 U/μL) 1 μL (see Note 11)
Milli-Q water To a final volume of 50 μL