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. Author manuscript; available in PMC: 2019 Jul 2.
Published in final edited form as: Methods Mol Biol. 2018;1708:645–663. doi: 10.1007/978-1-4939-7481-8_33
Component Amount
2× Reaction buffer 25 μL
5hmC dNTP Mix (10 mM each dNTP) 1 μL
5hmC control primer-F (10 μM) 2 μL
5hmC control primer-R (10 μM) 2 μL
pUC 19 vector (1 ng/–L) 1 μL
ZymoTaq™ DNA Polymerase (5 U/μL) 0.4 μL
Milli-Q water 18.6 μL
Final volume 50 μL