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. Author manuscript; available in PMC: 2019 Jul 2.
Published in final edited form as: Methods Mol Biol. 2018;1708:645–663. doi: 10.1007/978-1-4939-7481-8_33
Component Amount
Sheared DNA from step 3 1–3 μg
10 mM UDP-Glucose 0.4 μL
10× βGT protection buffer 2 μL
T4-βGT (10 U/μL) 2 μL
Milli-Q water To a final volume of 20 μL