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. Author manuscript; available in PMC: 2019 Jul 2.
Published in final edited form as: Methods Mol Biol. 2018;1708:645–663. doi: 10.1007/978-1-4939-7481-8_33
Component Amount
Milli-Q water To a final volume of 50 μL
Glucosylated DNA from step 6 300 ng
Tet oxidation reagent 2 15 μL
Tet oxidation reagent 1 3.5 μL (see Note 20)
mTet1 protein (5 mg/ ml) 4 μL (see Note 21)