Table 1. Toxicity of cubosome phytantriol formulations in various cell lines. Mass to volume ratios in µg/mL refer to total lipid and surfactant content.

Surfactant	Cell line	Reported toxicity	Assay	Ref.
F127	A549	Non-cytotoxic up to between 6.25 and 25 µg/mL, thereafter highly cytotoxic	Alamar Blue	[60,74]
	CHO-GFP	Non-cytotoxic up to between 6.25 and 25 µg/mL, thereafter highly cytotoxic	Alamar Blue	[60,74,146]
	HEK	Highly cytotoxic from 40 µg/mL	Alamar Blue	[146]
	L929	Cytotoxic from around 70 µg/mL	MTT	[77]
	ACHN	IC50 between 26 and 37 µg/mL	Alamar Blue	[48]
	LNCaP	IC50 between 9 and 12 µg/mL	Alamar Blue	[48]
	RBC from C57/BLK6 mice	Haemolytic at >1 µg/mL	Hemolysis	[48]
P(PEGA)5-50k	LNCaP	IC50 between 14 to 51 µg/mL	Alamar Blue	[48]
	ACHN	IC50 between 27 to 56 µg/mL	Alamar Blue	[48]
DSPE-PEG3.4 or 5k	A549	0% viability from between 12.5 & 25 µg/mL	Alamar Blue	[60]
	CHO-GFP	0% viability from between 12.5 & 25 µg/mL	Alamar Blue	[60]