. 2019 Apr 23;97(7):2989–3006. doi: 10.1093/jas/skz135

Table 9.

Effects of dietary soy isoflavones level and porcine reproductive and respiratory virus (PRRSV) infection on differential leukocyte counts and relative population proportions in weanling pigs

Item	Uninfected	PRRSV-infected				SEM	P-value
							Uninfected vs. infected control	Main effects²		Interaction
	Control¹	Control¹	Control + ISF¹	ETSBM¹	ETSBM + ISF¹			Soy Source	ISF	Soy Source × ISF
WBC, × 10³/µL
0 DPI	15.2	13.3	13.4	14.8	17.2	3.39	0.359	0.447	0.719	0.826
3 DPI	16.1	12.2	9.83	14.2	13.7	3.39	0.061	0.360	0.670	0.789
6 DPI	16.4	11.8*^a	12.7^a	24.4^b	14.5^a	3.24	0.031	0.026	0.153	0.018
14 DPI	16.6	23.1*	24.5	23.2	23.5	3.57	0.006	0.929	0.787	0.990
NEU, % of WBC
0 DPI	49.1	45.3	38.9	47.5	40.5	4.64	0.522	0.671	0.137	0.492
3 DPI	46.3	56.5	57.1	57.8	55.1	4.64	0.076	0.938	0.811	0.977
6 DPI	38.7	51.5*	54.2	59.1	47.4	4.42	0.030	0.345	0.301	0.281
14 DPI	35.7	49.9*	43.2	46.9	39.4	4.89	0.027	0.466	0.134	0.340
BAND, % of WBC
0 DPI	0.00	0.135	0.00	0.550	0.333	2.06	0.858	0.810	0.924	0.995
3 DPI	1.10	6.00*	1.10	6.63	3.18	2.06	0.038	0.412	0.012	0.073
6 DPI	0.304	6.11*	2.45	5.25	5.27	1.99	0.016	0.542	0.267	0.422
14 DPI	0.087	1.27	4.44	1.77	3.63	2.14	0.648	0.922	0.157	0.546
LYM, % of WBC
0 DPI	47.1	45.7	51.3	45.3	51.7	4.44	0.780	0.973	0.149	0.546
3 DPI	47.1	31.1*	35.8	31.0	37.5	4.43	0.002	0.851	0.179	0.588
6 DPI	52.9	32.2*	32.8	27.7	38.1	4.24	0.0001	0.905	0.176	0.348
14 DPI	51.7	39.5*	41.9	45.0	47.7	4.67	0.032	0.219	0.592	0.588
MONO, % of WBC
0 DPI	2.77	7.09*	7.19	5.56	6.00	1.22	0.016	0.229	0.842	0.379
3 DPI	4.47	3.25	2.10	2.98	2.62	1.22	0.472	0.911	0.508	0.905
6 DPI	6.06	6.50	7.53	5.42	5.62	1.17	0.804	0.181	0.594	0.530
14 DPI	4.73	4.81	5.86	3.70	5.07	1.28	0.963	0.454	0.307	0.679
EOSIN, % of WBC
0 DPI	1.19	1.63	2.11	1.14	1.42	0.979	0.644	0.446	0.591	0.830
3 DPI	0.993	2.25^ab	3.80^b	1.04^a	1.31^a	0.979	0.171	0.013	0.218	0.047
6 DPI	1.79	3.06	2.49	2.25	3.40	0.951	0.176	0.934	0.677	0.669
14 DPI	2.95	4.07	4.15	2.19	3.61	1.01	0.272	0.119	0.358	0.280
BASO, % of WBC
0 DPI	0.200	0.100	0.207	0.00	0.083	0.240	0.826	0.631	0.682	0.942
3 DPI	0.100	0.917	0.300	0.364	0.182	0.240	0.064	0.144	0.083	0.095
6 DPI	0.200	0.636	0.455	0.250	0.182	0.636	0.327	0.147	0.581	0.482
14 DPI	0.500	0.400	0.261	0.294	0.558	0.252	0.836	0.695	0.798	0.814

*Difference (P ≤ 0.05) between uninfected and infected groups fed the control diet.

^abMeans without common superscript letter differ at P ≤ 0.05.

Values represent least square means of 10 to 12 pigs. All pigs received allotted treatment diet starting −7 DPI. DPI = days postinoculation; ISF = isoflavones; WBC = white blood cells; NEU = neutrophils; BAND = immature neutrophils aka. band cells; LYM = lymphocytes; MONO = monocytes; EOSIN = eosinophils; BASO = basophils.

¹The following diet names have been assigned to a respective experimental diet groups: Control: soy protein concentrate + no supplemented ISF, fed to both uninfected and PRRSV-infected control pigs; Control + ISF: soy protein concentrate + supplemented ISF, fed to PRRSV-infected pigs only; ETSBM: ETSBM + no supplemented ISF, fed to PRRSV-infected pigs only; ETSBM + ISF: ETSBM + supplemented ISF, fed to PRRSV-infected pigs only.

²Main effect of day postinoculation (DPI); no 2- or 3-way interactive effects involving DPI were significant, so they were not included in the statistical model.