Table 2.
Sequence of primers used for the real-time quantitative PCR analysis
| Genes1 | Primer sequences (5′–3′)2 | Size (bp) | A T 3, °C | Accession number |
|---|---|---|---|---|
| CLDN-1 | F: GCCACAGCAAGGTATGGTAAC | 140 | 60 | NM_001258386.1 |
| R: AGTAGGGCACCTCCCAGAAG | ||||
| CLDN-2 | F: GCATCATTTCCTCCCTGTT | 156 | 60 | NM_001161638.1 |
| R: TCTTGGCTTTGGGTGGTT | ||||
| OCLN | F: CTACTCGTCCAACGGGAAAG | 158 | 62 | NM_001163647.2 |
| R: ACGCCTCCAAGTTACCACTG | ||||
| ZO-1 | F: CAGCCCCCGTACATGGAGA | 114 | 60 | XM_005659811.1 |
| R: GCGCAGACGGTGTTCATAGTT | ||||
| ZO-2 | F: ATTCGGACCCATAGCAGACATAG | 90 | 60 | NM_001206404.1 |
| R: GCGTCTCTTGGTTCTGTTTTAGC | ||||
| IGF-1 | F: CTGAGGAGGCTGGAGATGTACT | 137 | 58.5 | NM_001097417.1 |
| R: CCTGAACTCCCTCTACTTGTGTTC | ||||
| IGF-1R | F: GGGATGACGAGAGACATCTATGAG | 132 | 56.8 | NM_214172.1 |
| R: GAAGGACCAGACTCAGACTGC | ||||
| EGF | F: ATCTCAGGAATGGGAGTCAACC | 165 | 60 | NM_214020.1 |
| R: TCACTGGAGGATGGAATACAGC | ||||
| GLP-2 | F: ACTCACAGGGCACGTTTACCA | 149 | 56 | NM_005671883.1 |
| R: AGGTCCCTTCAGCATGTCTCT | ||||
| β-Actin | F: TCTGGCACCACACCTTCT | 114 | 57 | DQ178122 |
| R: TGATCTGGGTCATCTTCTCAC |
1 CLDN = claudin, OCLN = occludin, ZO-1 = zonula occludens 1, ZO-2 = zonula occludens 2, IGF-1R = IGF-1 receptor, EGF = epidermal growth factor, GLP-2 = glucagon-like peptide 2.
2F = forward primer; R = reverse primer.
3 A T = annealing temperature.