Table 3.
Sequence of primers and probes used for the real-time PCR analysis of microbial populations
| Primer | Nucleotide sequence (5′–3′)1 | Product size, bp | A T 2, °C | Reference |
|---|---|---|---|---|
| Total bacteria | F: ACTCCTACGGGAGGCAGCAG | 200 | 60 | Han et al. (2012) |
| R: ATTACCGCGGCTGCTGG | ||||
| Escherichia coli | F: CATGCCGCGTGTATGAAGAA | 96 | 60 | Qi et al. (2013) |
| R: CGGGTAACGTCAATGAGCAAA | ||||
| P: AGGTATTAACTTTACTCCCTTCCTC | ||||
| Lactobacillus | F: ACTCCTACGGGAGGCAGCAG | 126 | 60 | Qi et al. (2013) |
| R: CAACAGTTACTCTGACACCCGTTCTTC | ||||
| P: AAGAAGGGTTTCGGCTCGTAAAACTC-TGTT | ||||
| Bifidobacterium | F: CGCGTCCGGTGTGAAAG | 121 | 60 | Xiang et al. (2011) |
| R: CTTCCCGATATCTACACATTCCA | ||||
| P: ATTCCACCGTTACACCGGGAA | ||||
| Bacillus | F: GCAACGAGCGCAACCCTTGA | 92 | 60 | Qi (2011) |
| R: TCATCCCCACCTTCCTCCGGT | ||||
| P: CGGTTTGTCACCGGCAGTCACCT |
1F = forward primer; R = reverse primer; P = probe.
2 A T = annealing temperature.