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. 2019 Jul 2;10:2905. doi: 10.1038/s41467-019-10828-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Effect of various endocytosis inhibitors on ProTα- and (–30)GFP-mediated lipid nanoparticle delivery. Cationic lipid nanoparticles combined with either 10 nM ProTα-Cre or 100 nM (–30)GFP-Cre were incubated with HEK293-RFP cells that had been pre-treated with inhibitors that block various endocytosis pathways. After 2 day, delivery efficiency was measured via flow cytometry, and the percent of cells containing red fluorescence was quantified and normalized against a control sample lacking any inhibitor. Chloropramazine (CPZ) and wortmannin (WTM) modestly decreased the delivery efficiency, whereas MBCD strongly blocked Cre recombination. Values and error bars represent the mean and standard deviation of three independent biological replicates performed on different days. Source data are available in the Source Data file