Fig. 1.

Conditions of basal ISG expression. a, b Bone marrow-derived macrophages (BMDM) isolated from wild-type (WT), Stat1^−/−, Stat2^−/−, and Irf9^−/− mice were treated with 250 IU/ml of IFN-β as indicated. Gapdh-normalized gene expression was measured by RT-q-PCR. Data represent the mean and standard error of the mean (SEM) values of three independent experiments. P-values were calculated using the paired ratio t-test (*P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001). c Gene set enrichment analyses showing upregulation of an IFN and inflammatory response signature of untreated WT compared with untreated Irf9^−/− BMDM. The top correlated genes for each biological triplicate are displayed in the corresponding heat maps. The total height of the curve indicates the extent of enrichment (ES), with the normalized enrichment score (NES), the false discovery rate (FDR), and the P-value. Source data are provided as a source data file