Fig. 7.

Cross-regulation of ISGF3 subunits. a BMDMs isolated from WT, Stat1^−/−, Stat2^−/−, and Irf9^−/− mice were left untreated or treated with 15 µM P6 inhibitor for 3 h. Gapdh-normalized gene expression was measured by RT-q-PCR. Data represent relative expression in percent, where WT untreated equals 100%. Data represent the mean and standard error of the mean (SEM) values of three independent experiments. P-values were calculated using the paired ratio t-test (*P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001). b STAT1 (S1), STAT2 (S2), and IRF9 binding at the Stat1, Stat2, and Irf9 promoters (scale 0–150). The browser tracks represent data derived from the ChIP-seq experiments in BMDM described in the legend of Fig. 2. c Whole-cell extracts from wild-type, Stat1^−/−, Stat2^−/−, and Irf9^−/− BMDMs were tested by western blot for total STAT1, STAT2, and IRF9 levels. d Whole-cell extracts from wild-type, Stat1^−/−, Stat2^−/−, and Irf9^−/− mouse embryonic fibroblasts were analyzed by western blot for total STAT1, STAT2, and IRF9 levels. e Stat1^−/− mouse embryonic fibroblasts were stably transduced with a doxycycline-inducible STAT1-myc construct. Whole-cell extracts from wild-type, Stat1^−/−, and reconstituted Stat1^−/− MEFs were tested by western blot for total STAT1, STAT2, and IRF9 levels in the absence and presence of doxycycline. f The representative blot in e was quantified using ImageJ. Relative intensities of the bands were normalized to their corresponding GAPDH levels. Data represent relative intensities in percent, where STAT1, STAT2, and IRF9 levels in untreated wild-type MEFs equal 100%. Source data are provided as a source data file