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. 2019 Jul 2;9:9554. doi: 10.1038/s41598-019-45840-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Survival fraction of bacterial species Microbacterium sp. isolate LEMMJ01 () and E. coli K12A15 strain () after increasing doses of (A) UV-A (365 nm), (B) UV-B (312 nm) and C. UV-C (254 nm) irradiations. For this, stationary growth phase cultures were normalized at the optical densities (O.D.) of 0.1 and 1 for Microbacterium sp and E. coli, respectively. The suspensions of Microbacterium sp. isolate LEMMJ01 and E. coli K12A15 strain were exposed to UV-A at a dose rate of 40 W/m², UV-B at 15 W/m² and UV-C at 2.5 W/m². Aliquots were withdrawn at different times and plated to determine survival. The asterisks represent a significant difference (p < 0.005) between the survival of Microbacterium sp. LEMMJ01 and E. coli K12A15 after each radiation dose, as assessed by the Student’s t-test. Error bars represent the standard deviation of the counts of at least three independent experiments.

Inline graphic — Survival fraction of bacterial species Microbacterium sp. isolate LEMMJ01 () and E. coli K12A15 strain () after increasing doses of (A) UV-A (365 nm), (B) UV-B (312 nm) and C. UV-C (254 nm) irradiations. For this, stationary growth phase cultures were normalized at the optical densities (O.D.) of 0.1 and 1 for Microbacterium sp and E. coli, respectively. The suspensions of Microbacterium sp. isolate LEMMJ01 and E. coli K12A15 strain were exposed to UV-A at a dose rate of 40 W/m², UV-B at 15 W/m² and UV-C at 2.5 W/m². Aliquots were withdrawn at different times and plated to determine survival. The asterisks represent a significant difference (p < 0.005) between the survival of Microbacterium sp. LEMMJ01 and E. coli K12A15 after each radiation dose, as assessed by the Student’s t-test. Error bars represent the standard deviation of the counts of at least three independent experiments.