Fig. 7.

GFP-PR₂₈ expression is associated with synaptic transmission-related genes dysregulation. a MA-plot of differentially expressed genes in the cerebellum of 5-month-old control and GFP-PR₂₈ heterozygous mice. Red blots indicate significant changes, n = 3, 3 mice. b Hierarchical clustering of differentially expressed genes in the cerebellum of 5-month-old control and GFP-PR₂₈ heterozygous mice. c Gene ontology (GO) biological processes analyses of upregulated and downregulated genes in (b). d The Reactome pathway analyses of top five enriched terms of downregulated genes in (b). Gene numbers indicate genes that are enriched in this pathway. Rich factor indicates the ratio of enriched genes to total genes in this pathway. e Relative mRNA expression of six genes (Camk4, Grin2a, Kcnj9, Rims3, Syt2, and Unc13a) in association with synaptic transmission in the cerebellum of 5-month-old control and GFP-PR₂₈ heterozygous mice. Two-tailed t test, n = 4, 4 mice. f Relative expressing levels of CaMK IV in the cerebellum of 6-month-old control and GFP-PR₂₈ heterozygous mice, determined by western blotting assay. Two-tailed t test, CaMK IV, n = 5, 5 mice. All data are displayed as mean ± s.e.m. *P < 0.05, **P < 0.01, ****P < 0.0001