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. 2019 Jul 2;9:9520. doi: 10.1038/s41598-019-45963-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Serial cross-sections imaged using focused ion beam/scanning electron microscopy tomography for the experimental group in horizontal fibre area. White circles and ellipses represent regions of intercellular contact. Magenta and green regions represent regions of cell contact with other cells. In serial cross-sections, regions of the cells in direct contact with surrounding cells overlapped. The region of intercellular contact was large, ranging from the bone to the cementum. Magenta and green regions in slices 514–526 are separate areas. The two areas in slice 537 are overlapping and the overlapping region is expanded. ab, alveolar bone; PDL, periodontal ligament; c, cementum. Scale bars: 10 μm.