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. 2019 Jul 2;9:9559. doi: 10.1038/s41598-019-45966-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Z-FL does not reduce cell viability or cause cell death in vitro in human corneal epithelial (HCE-T) cells. (A) The percentage of cell viability at all doses tested of Z-FL is plotted relative to that seen with KSFM treatment (cultured medium) and saponin treatment. P = 0.04 (20 µM vs saponin), P = 0.02 (100 µM vs saponin), P = 0.046 (200 µM vs saponin), and P = 0.007 (KSFM vs saponin); (B) The percentage of cell death at all doses of Z-FL tested is plotted relative to saponin treatment, and KSFM treatment (cultured medium). P = 0.002 (20 µM vs saponin), P = 0.002 (100 µM vs saponin), P = 0.004 (200 µM vs saponin), and P = 0.005 (KSFM vs saponin). Cells treated with KSFM were positive controls for live cells, while cells treated with saponin were positive controls for dead cells. N = 3 wells/group, data shown as mean ± SD, and a one-way ANOVA with Tukey’s multiple comparison was used to compare between treatment groups).