Table 3.
Quantification of metabolites detected by 1H-NMR of the ten cell-free supernatants produced by Lactobacillus spp. grown in MRS with glycerol (20% v/v) expressed as mmoL.a
| VV1 | RB2 | ML4 | SA2 | SL4 | SA4 | SA1 | AD4 | AD2 | ML1 | |
|---|---|---|---|---|---|---|---|---|---|---|
| Metabolites | ||||||||||
| Organic acids (alcohols, aldehydes, and ketones) | ||||||||||
| 1.3-Dihydroxyacetone | 0.1160 | 0.0914 | –b | 0.1733 | 0.0667 | 0.1601 | 0.1243 | 0.0333 | – | – |
| Acetate | – | – | 11.6086 | – | – | – | – | – | – | 5.4060 |
| Acetoin | – | – | 0.0153 | 0.1002 | – | 0.0311 | – | 0.0935 | 0.4773 | 0.0572 |
| Acetone | 0.0404 | 0.0374 | 0.0225 | 0.0371 | 0.0337 | 0.0288 | – | 0.0468 | 0.0263 | 0.0049 |
| Ethanol | 1.1489 | 0.3767 | 49.1248 | 0.4433 | 5.1998 | 0.5562 | 12.2024 | 0.7748 | 0.4050 | 10.6746 |
| Formate | – | 0.0051 | 0.4260 | – | – | 0.0249 | – | 0.0037 | 0.0661 | 0.1656 |
| Lactate | 0.1762 | 0.3521 | 6.7589 | – | 0.4303 | – | – | 0.6278 | – | – |
| Pyruvate | 0.0609 | 0.0298 | – | 0.0350 | – | – | – | 0.0014 | 0.1049 | 0.0133 |
| Nucleosides and nucleotides | ||||||||||
| Cytosine | 0.0098 | 0.0080 | – | 0.0258 | 0.0103 | 0.0365 | 0.0043 | – | – | – |
| Uracil | 0.0913 | 0.1011 | 0.0697 | 0.1575 | 0.1214 | 0.1535 | 0.1128 | 0.1027 | 0.0624 | 0.0962 |
| Uridine | 0.0051 | 0.0049 | – | – | – | – | – | 0.0058 | 0.0157 | – |
| Amino acids and derivatives | ||||||||||
| Phenylalanine | – | 0.0785 | – | 0.1060 | 0.0663 | 0.0629 | 0.0432 | 0.0437 | – | – |
| Tryptophan | – | – | 0.0163 | 0.0209 | – | 0.0252 | 0.0391 | 0.0100 | 0.0136 | 0.0373 |
| Tyrosine | 0.2713 | 0.2863 | 0.2905 | 0.3510 | 0.2664 | 0.3558 | 0.3111 | 0.2579 | 0.3416 | 0.3533 |
| Alanine | 0.3561 | 0.3899 | 0.6423 | 0.8967 | 0.4111 | 0.7354 | 0.7214 | 0.2853 | 0.8579 | 0.8608 |
| Asparagine | 0.0414 | 0.0577 | 0.2247 | 0.0852 | 0.0754 | 0.0030 | 0.0880 | 0.0852 | 0.1728 | 0.1414 |
| Aspartate | 0.0332 | 0.0417 | 0.3168 | 0.0214 | 0.0314 | 0.0836 | 0.0357 | 0.0234 | 0.2074 | 0.2043 |
| Betaine | 0.2029 | 0.3092 | – | 0.0917 | 0.2443 | 0.0931 | – | 0.3463 | 0.0172 | – |
| Isoleucine | 0.0613 | 0.1082 | 0.1213 | 0.2106 | 0.0439 | 0.2016 | 0.1212 | 0.0224 | 0.1306 | 0.2062 |
| Methionine | 0.0075 | 0.0136 | – | 0.0584 | – | 0.0411 | 0.0051 | – | – | – |
| Pyroglutamate | 0.3944 | 0.3923 | 0.5473 | 0.8159 | 0.5996 | 0.7930 | 0.7598 | 0.5096 | 0.8496 | 0.6239 |
| Sarcosine | 0.0453 | 0.0421 | 0.0446 | 0.0578 | 0.0489 | 0.0538 | 0.0171 | 0.0357 | 0.0280 | 0.0392 |
| Threonine | 0.2956 | 0.2910 | 0.9926 | 0.4964 | 0.2264 | 0.4695 | 0.4104 | 0.1056 | 0.7721 | 0.8477 |
| Valine | 0.2742 | 0.2156 | 0.2496 | 0.5151 | 0.2613 | 0.4545 | 0.3531 | 0.1043 | 0.2749 | 0.4875 |
| Sugars and derivates | ||||||||||
| Fructose | 0.4516 | 0.4468 | – | 0.0847 | 0.5549 | 0.2224 | – | 0.5762 | 0.2541 | – |
| Glucose | 7.1884 | 10.3974 | – | 3.0722 | 8.5333 | 4.0134 | 1.0097 | 11.6231 | 3.3199 | – |
| Maltose | 0.1253 | 0.1195 | – | – | 0.1396 | – | 0.1020 | 0.1604 | 0.1925 | – |
| Mannitol | – | – | – | – | – | – | – | 0.0761 | – | – |
| Ribose | 0.0171 | 0.0192 | – | 0.0075 | 0.0151 | 0.0107 | – | 0.0247 | 0.0105 | – |
| Others | ||||||||||
| 3-Hydroxyphenylacetate | 0.0690 | 0.0485 | 0.0900 | 0.0857 | 0.0757 | 0.0904 | 0.0326 | 0.0575 | 0.0567 | 0.1078 |
| Choline | 0.0072 | 0.0115 | 0.0112 | 0.0172 | 0.0117 | 0.0180 | – | 0.0117 | 0.0071 | – |
| 2,3-Butanediol | 0.0165 | 0.0042 | 0.2947 | 0.0070 | 0.0241 | 0.0065 | 1.2774 | 0.0060 | 0.0108 | 0.2168 |
aReferred to the quantities of substances found in the samples after subtracting the quantities of the components present in the MRS culture medium reported in Table 4; bThe value of quantification was negative after subtracting the quantities of the components of MRS culture medium.