Table 4.
Composition of 69966 MRS Broth (Lactobacillus Broth acc. to De Man, Rogosa, and Sharpe) Datasheet Sigma-Aldrich Co.a
| Ingredients | Grams/Liter |
|---|---|
| Peptone | 10.0 |
| Meat extract | 8.0 |
| Yeast extract | 4.0 |
| D-(+)-Glucose | 20.0 |
| Dipotassium hydrogen phosphate | 2.0 |
| Sodium acetate trihydrate | 5.0 |
| Triammonium citrate | 2.0 |
| Magnesium sulfate heptahydrate | 0.2 |
| Manganous sulfate tetrahydrate | 0.05 |
| 1H-NMR detection | mmoL |
| 1.3-Dihydroxyacetone | 0.0752 |
| Acetate | 36.7000 |
| Acetoin | 0.0248 |
| Acetone | 0.0116 |
| Ethanol | 0.0386 |
| Formate | 0.1120 |
| Lactate | 3.0400 |
| Pyruvate | 0.0581 |
| Cytosine | 0.0294 |
| Uracil | 0.1350 |
| Uridine | 0.0939 |
| Phenylalanine | 1.4000 |
| Tryptophan | 0.2440 |
| Tyrosine | 0.2281 |
| Alanine | 2.3300 |
| Asparagine | 0.1240 |
| Aspartate | 0.1930 |
| Betaine | 0.9820 |
| Isoleucine | 0.9150 |
| Methionine | 0.3370 |
| Pyroglutamate | 0.4340 |
| Sarcosine | 0.2500 |
| Threonine | 0.4360 |
| Valine | 1.5600 |
| Fructose | 0.2020 |
| Glucose | 8.4600 |
| Maltose | 0.1590 |
| Mannitol | 6.2500 |
| Ribose | 0.0346 |
| 3-Hydroxyphenylacetate | 0.1020 |
| Choline | 0.0786 |
| Propylene Glycol | 0.0121 |
aFinal pH 6.2 ± 0.2 at 25°C.