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. 2019 Jun 26;11:156. doi: 10.3389/fnagi.2019.00156

Figure 4.

Figure 4

Age-related decline in utricular ribbon synapse density is restricted to the extrastriolar region and occurs only at 24 months of age. (A–E) Extrastriolar and (F–J) striolar regions of 2, 18 and 24 month old mouse utricles were analyzed separately after immunostaining with GluA2 and Ctbp2. (A) Representative confocal images of extrastriolar ribbon synapses. (B,C) Density of synaptic ribbon and (D,E) putative ribbon synapses either normalized to unit area (B,D) or each hair cell (C,E) in 2 (n = 8), 5 (n = 12), 18 (n = 12) and 24 (n = 9) month old utricular extrastriola. *p < 0.05 and ***p < 0.001 by one-way ANOVA comparing to 2-month-old group followed by Dunn’s post-test. (F) Representative confocal images of striolar ribbon synapses. (G,H) Density of synaptic ribbon and (I,J) putative ribbon synapses either normalized to unit area (G,I) or each hair cell (H,J) in 2 (n = 8), 5 (n = 6), 18 (n = 12) and 24 (n = 9) month old utricular striola.