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. 2019 Jun 26;11:156. doi: 10.3389/fnagi.2019.00156

Figure 7.

Figure 7

Loss of calyceal synapses but not bouton synapses in 24-month-old utricular extrastriola. (A–E) Extrastriolar and (F–J) striolar regions of 2, 18 and 24-month-old mouse utricles were analyzed separately after immunostaining with tenascin-C (Tnc) and Ctbp2. (A) Representative confocal images of extrastriolar calyces and synaptic ribbons. Density of total synaptic ribbon (B), noncalyceal ribbon (C) and calyceal ribbon (D) in 2 (n = 13), 18 (n = 8) and 24 (n = 7) month old utricular extrastriola. (E) Number of synaptic ribbons associated with each calyx in 2 (n = 13), 18 (n = 8) and 24 (n = 7) month old utricular extrastriola. *p < 0.05 and **p < 0.01 by one-way ANOVA comparing to 2-month-old group followed by Dunn’s post-test. (F) Representative confocal images of striolar calyces and synaptic ribbons. Density of total synaptic ribbon (G), noncalyceal ribbon (H) and calyceal ribbon in (I) 2 (n = 14), 18 (n = 7) and 24 (n = 9) month old utricular striola. (J) Number of synaptic ribbons associated with each calyx in 2 (n = 14), 18 (n = 7) and 24 (n = 9) month old utricular striola.