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. 2019 Jun 26;13:65. doi: 10.3389/fnana.2019.00065

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Copyright © 2019 Redlich and Lim.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Evaluation of the g-ratio in the deeper cortex by THG. (A) The cross sections of myelinated axons (left) and the corresponding intensity profiles (right). The position of adaxonal and abaxonal membranes can be precisely determined (arrowheads, right). (B) The g-ratio vs. the axon diameter for a total of 20 axons in layer 6 of the primary motor cortex (M1) of C57BL/6 mice (N = 5). The dashed line is for the case of a 0.5-μm myelin sheath. (C) A single internode at a depth of approximately 500 μm exhibiting variations of the g-ratio across the length between arrowheads (yellow). The dashed line denotes the average g-ratio. Scale bar, 5 μm.